Technical Reference #447

447.

Modulation Of Calcium Responses By Altered Peptide Ligands In A Human T Cell Clone Yu-Zhen Chen; Zhong-Fang Lai; Katsuhide Nishi and Yasuharu Nishimura, Kumamoto University, European Journal of Immunology, 28(447), (1998)
Link To Paper

Abstract:
To determine whether altered peptide ligands (APL) affect calcium signaling events weinvestigated changes in intracellular calcium concentration ([Ca2+]i) in human T cell clonestimulated with either the fully agonistic peptide M12p54–68 the partiall

Keywords:
Human T cell; T cell activation; Altered peptide ligand; Calcium response; HLA-DR

Materials & Methods:
For video imaging experiments [51] T cells (5 × 105 cells/ml) were incubated with 3.5 ? M fura-2/AM (Dojindo Laboratories Kumamoto) for 45 min at 37 °C then the cells were washed four times with the medium. To prevent movement of T cells during the

Microscopic Technique
Inverted Microscopy

Cell Type(s)
T