Technical Reference #446

446.

Real-Time Imaging Of Gene Promoter Activity Using An Adenoviral Reporter Construct Demonstrates Transcriptional Dynamics In Normal Anterior Pituitary Cells J A Stirland; Z C Seymour; S Windeatt; A J Norris; P Stanley; M G Castro; A S I Loudon; M R H White and J R E Davis, University of Manchester, Journal of Endocrinology, 178(446), (2003)
Link To Paper

Abstract:
Although analysis of luciferase activity using luminescence imaging has provided new insights into the dynamicregulation of gene expression in living tissues studiesin vitro have relied on stably transfected clonal cell lineslimiting the choice o

Keywords:
gene promoter activity; recombinant adenovirus; gene regulation

Materials & Methods:
Imaging experiments were performed as previously described (Takasuka et al. 1998 McFerran et al. 2001 Norris et al. 2003). Cells were seeded (5 104 and 1 105 cells/dish for GH3 and primary PD cells respectively) onto 35 mm coverslip dishes (MatTek C

Microscopic Technique
Luminescence Microscopy Live-cell imaging; icroscopy

Cell Type(s)
GH3