Technical Reference #443

443.

Functional Inactivation Of Immature Dendritic Cells By The Intracellular Parasite Toxoplasma Gondii Amy S. McKee; Florence Dzierszinski; Marianne Boes; David S. Roos; and Edward J. Pearce, University of Pennsylvania, The The Journal of Immunology, 173(443), (2004)
Link To Paper

Abstract:
Despite its noted ability to induce strong cellular immunity and its known susceptibility to IFN- -dependent immune effectormechanisms the protozoan Toxoplasma gondii is a highly successful parasite able to replicate disseminate and either kill the

Keywords:
Toxoplasma gondii; parasite; immune

Materials & Methods:
DC (2 106/ml) were cultured on sterile glass coverslips in 24-well plates with or without live or HK tachyzoites or soluble extracts for 18 h. Cells were fixed in 4% paraformaldehyde permeabilized in saponin buffer and stained with biotin-conjuga

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Dendritic