Technical Reference #442

442.

Preferential Inhibition By A Novel Na1/Ca21 Channel Blocker Ns-7 Of Severe To Mild Hypoxic Injury In Rat Cerebrocortical Slices: A Possible Involvement Of A Highly Voltage-Dependent Blockade Of Ca21 Channel MICHIKO OKA; YOSHINORI ITOH; and YOJIRO UKAI, Nippon Shinyaku Co Ltd, Journal of Pharmacology and Experimental Therapeutics, 293(442), (2000)
Link To Paper

Abstract:
The hypoxic injury was induced in rat cerebrocortical slices bythe exposure to hypoxia for 45 min in the absence or presenceof 3 mM glucose followed by reoxygenation for 5 h. The injurywas more pronounced in the absence of glucose (severe hypoxic

Keywords:
Hypoxia; glucose; Ca2+ channels; rat cerebrocortical slices

Materials & Methods:
Primary neuronal culture was prepared from the cerebral cortex of fetal mice as described previously (Ma et al. 1991; Tatsumi et al. 1998a). Briefly the cerebral cortex was dissected from 15-day-old fetal mouse brain and the meninges were carefull

Microscopic Technique
Inverted Microscopy

Cell Type(s)
NG108-15