Technical Reference #441

441.

Loss Of The Homotypic Fusion And Vacuole Protein Sorting Or Golgi-Associated Retrograde Protein Vesicle Tethering Complexes Results In Gentamicin Sensitivity In The Yeast Saccharomyces Cerevisiae Mark C. Wagner; Elizabeth E. Molnar; Bruce A. Molitoris; and Mark G. Goebl, Indiana Center for Biological Microscopy, Antimicrobial Agents and Chemotherapy, 50(441), (2006)
Link To Paper

Abstract:
Gentamicin continues to be a primary antibiotic against gram-negative infections. Unfortunately associatednephro- and ototoxicity limit its use. Our previous mammalian studies showed that gentamicin is trafficked tothe endoplasmic reticulum in a retr

Keywords:
Gentamicin; toxicity; Golgi-associated retrograde protein (GARP); aminoglycoside; yeast Saccharomyces cerevisiae

Materials & Methods:
TR was conjugated to gentamicin as previously described (32). Yeasts were incubated with TR-gentamicin or lucifer yellow (LY) for the specified period of time under the same growth conditions as those used for the growth assay. LY accumulates in the ye

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Yeast