Technical Reference #439

439.

The Actin-Binding Domain Of Slac2-A/Melanophilin Is Required For Melanosome Distribution In Melanocytes Taruho S. Kuroda; Hiroyoshi Ariga; and Mitsunori Fukuda, RIKEN, Molecular and Cellular Biology, 23(439), (2003)
Link To Paper

Abstract:
Melanosomes containing melanin pigments are transported from the cell body of melanocytes to the tips oftheir dendrites by a combination of microtubule- and actin-dependent machinery. Three proteins Rab27Amyosin Va and Slac2-a/melanophilin (a linke

Keywords:
Melanocytes; Griscelli syndrome; pigment

Materials & Methods:
Melan-a cells (105 cells [the day before transfection] per 35-mm-diameter glass-bottom dish; Mat- Tek Corp.) were transfected with 2 g of a plasmid encoding a green fluorescent protein (GFP)-tagged Slac2-a protein with 3 l of Fugene 6. At 36 to 48 h

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7