Technical Reference #438

438.

Cell Cycle Behavior Of Human Hp1 Subtypes: Distinct Molecular Domains Of Hp1 Are Required For Their Centromeric Localization During Interphase And Metaphase Tomohiro Hayakawa; Tokuko Haraguchi; Hiroshi Masumoto and Yasushi Hiraoka, Kansai Advanced Research Center, The Journal of Cell Biology, 116(438), (2003)
Link To Paper

Abstract:
Heterochromatin protein 1 (HP1) plays an important rolein heterochromatin formation. Three subtypes of HP1namely HP1a b and g have been identified in humans. Inthis study using yellow fluorescent protein (YFP) fusionconstructs we examined th

Keywords:
Heterochromatin; HP1; PML; Centromere; CENP-B

Materials & Methods:
Transfection reactions were carried out with LipofectAmine PLUS (Invitrogen). HeLa cells cultured in a 35 mm glass-bottom culture dish (MatTek Ashland MA USA) were transfected with 250 ng of plasmid DNA and incubated at 37°C for 1.5 hours. For cotr

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa