Technical Reference #436

436.

Real Time Fluorescence Imaging Of Plctranslocation And Its Interaction With The Epidermal Growth Factor Receptor Miho Matsuda; Hugh F. Paterson; Rosie Rodriguez; Amanda C. Fensome; Moira V. Ellis; Karl Swann; and Matilda Katan, The Institute of Cancer Research, The Journal of Cell Biology, 153(436), (2001)
Link To Paper

Abstract:
The translocation of fluorescently taggedPLCgand requirements for this process in cells stimulatedwith EGF were analyzed using real time fluorescencemicroscopy applied for the first time to monitorgrowth factor receptor–effector interactions.

Keywords:
PLC; EGF receptor; translocation; real time imaging; SH2 and PH domains

Materials & Methods:
A431 and COS-7 cells were cultured in DME supplemented with 10% FBS. The transfection of GFP or RFP fusion protein constructs were made using cells in a 60-mm dish (Nunc) a glass-bottomed 50-mm dish (MatTek Corporation) or on 22-mm-diameter coverslip

Microscopic Technique
Confocal Microscopy

Cell Type(s)
A431