Technical Reference #435

435.

Lfa-1/Icam-1 Interaction Lowers The Threshold Of B Cell Activation By Facilitating B Cell Adhesion And Synapse Formation Yolanda R. Carrasco; Sebastian J. Fleire; Thomas Cameron; Michael L. Dustin; and Facundo D. Batista, Lincoln's Inn Fields Laboratories, Immunity, 20(435), (2004)
Link To Paper

Abstract:
Theintegrin LFA-1 and its ligand ICAM-1 mediateB cell adhesion but their role in membrane-bound antigen recognitionis still unknown. Here using planar lipid bilayersand cells expressing ICAM-1 fused to green fluorescence protein we found that the

Keywords:
Cell Adhesion; synapse; B cell receptor (BCR)

Materials & Methods:
Time-lapse video microscopy was performed using a Nikon Diaphot 200 inverted microscope equipped with a CCD camera (Hama-matsu Orca). ICAM-1-GFP APCs were split on 35 mm glass-bottom dishes (MatTek Corporation Ashland MA). The dish was placed on a 37 C

Microscopic Technique
Video Microscopy, Time Lapse

Cell Type(s)
Murine L cells fibroblasts