Technical Reference #434

434.

Mechanisms For Focusing Mitotic Spindle Poles By Minus End–Directed Motor Proteins Gohta Goshima; François Nédélec; and Ronald D. Vale, HHMI, The Journal of Cell Biology, 171(434), (2005)
Link To Paper

Abstract:
During the formation of the metaphase spindle inanimal somatic cells kinetochore microtubulebundles (K fibers) are often disconnected fromcentrosomes because they are released from centrosomesor directly generated from chromosomes. Tocreate th

Keywords:
spindle poles; K fibers; mitosis; dynein

Materials & Methods:
Drosophila Schneider cell line (S2) was cultured and RNAi was performed according the methods of Clemens et al. (2000). Templates for in vitro transcription were generated by PCR using the primers described in Table S1 (available at http://www.jcb.org/

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence, Time-Lapse Microscopy

Cell Type(s)
Drosophila