Technical Reference #432

432.

Missense Mutations In The Globular Tail Of Myosin-Va In Dilute Mice Partially Impair Binding Of Slac2-A/Melanophilin Mitsunori Fukuda and Taruho S. Kuroda, RIKEN, The Journal of Cell Science, 117(432), (2004)
Link To Paper

Abstract:
The well-known coat-color mutant mouse dilute exhibits adefect in melanosome transport and although variousmutations in the myosin-Va gene which encodes an actinbasedmotor protein have been identified in dilute micewhy missense mutations in th

Keywords:
Slac2-a/melanophilin; Myosin-Va; Rab27A; Melanosome transport; Griscelli syndrome

Materials & Methods:
Cells of a murine immortalized melanocyte cell line melan-a cells (generous gift of Dorothy C. Bennett St George’s Hospital Medical School London UK and Katsuhiko Tsukamoto University of Yamanashi Yamanashi Japan) were cultured on glass-bottom

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7