Technical Reference #431

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass coverslip MatTek microwell dishes (MatTek Corp.; Ashland; MA)

431.

Novel Fluorescence-Based Approaches For The Study Of Biogenic Amine Transporter Localization; Activity; And Regulation J.N. Mason; H. Farmer; I.D. Tomlinson; J.W. Schwartz; V. Savchenko; L.J. DeFelice; S.J. Rosenthal; R.D. Blakely, Vanderbilt University, The Journal of Neuroscience Methods, 143(431), (2005)
Link To Paper

Abstract:
Pre-synaptic norepinephrine (NE) and dopamine (DA) transporters (NET and DAT) terminate catecholamine synaptic transmission throughreuptake of released neurotransmitter. Recent studies reveal that NET and DAT are tightly regulated by receptor and second

Keywords:
Catecholamine; Dopamine; Norepinephrine; ASP+; Quantum dots

Materials & Methods:
To assess specific labeling of SA-conjugated nanocrystals we use HEK293 cells that stably express the cloned hNET transporter (Galli et al. 1995). The cell culture is performed as described in Section 2.2.1. To assess the specific labeling of the An

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293