Technical Reference #430

430.

Cdc25B Cooperates With Cdc25A To Induce Mitosis But Has A Unique Role In Activating Cyclin B1–Cdk1 At The Centrosome Arne Lindqvist; Helena Källström; Andreas Lundgren; Emad Barsoum; and Christina Karlsson Rosenthal, Karolinska Institutet, The Journal of Cell Biology, 171(430), (2005)
Link To Paper

Abstract:
Cdc25 phosphatases are essential for the activationof mitotic cyclin–Cdks but the precise roles of thethree mammalian isoforms (A B and C) are unclear.Using RNA interference to reduce the expression ofeach Cdc25 isoform in HeLa and HEK293 cells

Keywords:
mitosis; cyclin B1–Cdk1; phosphorylation

Materials & Methods:
Cells growing on a glass-bottomed dish (MatTek) or a coverslip were microinjected with 10 nM siRNA and 0.008 g/ l plasmid CFP (pCFP)- Golgi as a marker in the first release of a double thymidine block. As for time-lapse microscopy different si

Microscopic Technique
Time Lapse Microscopy, Three-Dimensional

Cell Type(s)
HeLa