Technical Reference #429
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
glassbottom culture dish (MatTek Co.; Ashland; MA) 
429. |
Tacrolimus Impairment Of Insulin Secretion In Isolated Rat Islets Occurs At Multiple Distal Sites In Stimulus-Secretion Coupling
YUJI UCHIZONO; MASANORI IWASE; UDAI NAKAMURA; NOBUHIRO SASAKI; DAISUKE GOTO; AND
MITSUO IIDA,
Kyushu University,
Endocrinology,
145(429),
(2004)
Link To Paper
Abstract:
Tacrolimus causes posttransplant diabetes mellitus althoughthe pathogenetic mechanisms remain controversial. We studiedthe mechanism of tacrolimus-induced impairment of insulinsecretion using isolated rat pancreatic islets. Tacrolimuscaused reduc Keywords:
Insulin Secretion; Tacrolimus; glucosestimulated
insulin secretion; graft survival; organ transplantation; nuclear factor of activated T
cells (NFAT); calcineurin Materials & Methods:
Measurements (n 5) were carried out in Krebs-Ringer bicarbonate
buffer containing 128.8 mmol/liter NaCl 4.8 mmol/liter KCl 1.2 mmol/
literMgSO4 1.2 mmol/literKH2PO4 2.5 mmol/liter CaCl2 5 mmol/liter
NaHCO3 and 10 mmol/liter HEPES (KRBH; equilib Microscopic Technique
Fluorescence Microscopy Cell Type(s)
Islet |
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