Technical Reference #428

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed culture dishes (35-mm uncoated dishes; 1 cm diameter) from MatTek (Ashland; MA; USA)

428.

Immobilized Culture Of Nonadherent Cells On An Oleyl Poly(Ethylene Glycol) Ether-Modified Surface Koichi Kato; Kohei Umezawa; Daniel P. Funeriu; Masato Miyake; Jun Miyake; and Teruyuki Nagamune, National Institute of Advanced Industrial Science and Technology, BioTechniques, 35(428), (2003)
Link To Paper

Abstract:
Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent the biocompatible anchor for me

Keywords:
nonadherent cells; biocompatible anchor for membrane (BAM); cell adhesion

Materials & Methods:
Bovine serum albumin (BSA; low endotoxin) was purchased from Sigma (St. Louis MO USA) and phosphate-buffered saline (PBS) Dulbecco’s modified Eagle medium (DMEM) and RPMI 1640 culture medium were purchased from NISSUI Pharmaceutical Ltd. (Tokyo Jap

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
3T3