Technical Reference #427

427.

Identification Of T Cell Death-Associated Gene 8 (Tdag8) As A Novel Acid Sensing G-Protein-Coupled Receptor Satoshi Ishii; Yasuyuki Kihara; and Takao Shimizu, University of Tokyo, The Journal of Biological Chemistry, 280(427), (2005)
Link To Paper

Abstract:
T cell death-associated gene 8 (TDAG8) is a G-proteincoupled receptor mainly expressed in lymphoid organsand cancer tissues. TDAG8 shares high amino acid sequencehomologies with recently reported proton-sensingG-protein-coupled receptors G2A OGR1

Keywords:
Gene 8 (TDAG8); galactosylceramidase; Cell Death

Materials & Methods:
Cells were seeded onto a collagen-coated glass-bottomed 3.5-cm dish (MatTek Corp. Ashland MA) and serumstarved for 18 h. Following stimulation with DMEM at pH 6.4 and 8.1 containing 0.1% BSA for 15 min at 37 °C the cells were fixed with PBS containi

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
CHO