Technical Reference #426

426.

Novel Dynamic Rheological Behavior Of Individual Focal Adhesions Measured Within Single Cells Using Electromagnetic Pulling Cytometry Darryl R. Overby; Benjamin D. Matthews; Eben Alsberg; Donald E. Ingber, Children's Hospital Boston, Acta Biomaterialia, 1(426), (2005)
Link To Paper

Abstract:
The rheology of cells and sub-cellular structures such as focal adhesions are important for cell form and function. Here wedescribe electromagnetic pulling cytometry (EPC) a technique to analyze cell rheology by applying dynamic tensional forces to

Keywords:
Cell mechanics; Cytoskeleton; Focal adhesion; Creep; Magnetic

Materials & Methods:
Bovine capillary endothelial cells were cultured in 10% CO2 at 37 C on gelatin-coated tissue culture dishes in Dulbeccos modified Eagles medium (DMEM Gibco-BRL) supplemented with 10% calf serum (CS; Hyclone) 10 mM Hepes (JRH-Biosciences) 2 mM L

Microscopic Technique
Scanning Probe Microscopy, Atomic Force Microscopy (AFM)

Cell Type(s)
BCE