Technical Reference #425

425.

Identification Of Novel Principles Of Keratin Filament Network Turnover In Living Cells Reinhard Windoffer; Stefan Wo¨ ll; Pavel Strnad; and Rudolf E. Leube, Johannes Gutenberg University, Molecular Biology of the Cell, 15(425), (2004)
Link To Paper

Abstract:
It is generally assumed that turnover of the keratin filament system occurs by exchange of subunits along its entire lengththroughout the cytoplasm. We now present evidence that a circumscribed submembranous compartment is actually themain site for ne

Keywords:
keratin; intermediate filaments; Keratin filaments

Materials & Methods:
For time-lapse fluorescence microscopy of living cells phenol-red–free Hanks’ medium (Invitrogen GmbH) was used. Images were recorded by epifluorescence microscopy using inverse optics (Olympus Hamburg Germany) and an attached IMAGO slow-scan CCD ca

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
Ptk2