Technical Reference #423

423.

K-Space Image Correlation Spectroscopy (Kics): A Method For Accurate Transport Measurements Independent Of Fluorophore Photophysics David L. Kolin; David Ronis; and Paul W. Wiseman, McGill University, Biophysical Journal, 91(423), (2005)
Link To Paper

Abstract:
We present the theory and application of reciprocal space image correlation spectroscopy (kICS).This technique measures the number density diffusion coefficient and velocity of fluorescentlylabeled macromolecules in a cell membrane imaged on a confo

Keywords:
image correlation spectroscopy; light scattering theory; fluorescence correlation spec- troscopy; membrane dynamics; reciprocal space; photobleaching; fluorophore blinking

Materials & Methods:
CHO-K1 cells were transfected to express 5-integrin/eGFP as previously described (28) (provided by A.R. Horwitz Univ. of Virginia). Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum 4 mM L-glutamine

Microscopic Technique
Fluorescence Microscopy, Total Internal Reflection Fluorescence Microscopy

Cell Type(s)
CHO