Technical Reference #420

420.

Rab11A And Myosin Vb Are Required For Bile Canalicular Formation In Wif-B9 Cells Yoshiyuki Wakabayashi*‡; Parmesh Dutt; Jennifer Lippincott-Schwartz*; and Irwin M. Arias, National Institutes of Health, PNAS, 102(420), (2005)
Link To Paper

Abstract:
Hepatocytes polarize by forming functionally distinct sinusoidal(basolateral) and canalicular (apical) plasma membrane domains.Two distinct routes are used for delivery of membrane proteins tothe canaliculus. Proteins having glycosylphosphatidylinos

Keywords:
hepatocyte polarization; plasma membrane segregation

Materials & Methods:
WIF-B9 cells were cultured in a humidified 7% CO2 incubator at 37°C (14). Unless otherwise specified cells were plated on a 35-mm glass bottom dish (MatTek) at 1.4 105 cells per dish in complete medium. Infections with recombinant adenoviruses enco

Microscopic Technique
Confocal Microscopy

Cell Type(s)
WIF-B9