Technical Reference #419

419.

Diacylglycerol And Protein Kinase D Localization During T Lymphocyte Activation Martin Spitaler; Elisabeth Emslie; C. David Wood; and Doreen Cantrell, University of Dundee, Immunity, 24(419), (2006)

Abstract:
The serine kinase protein kinase D (PKD) has a cysteine-rich domain (CRD) that binds diacylglycerol (DAG)with high affinity. PKD is cytosolic in unstimulated Tcells but it rapidly polarizes to the immunological synapsein response to antigen/antig

Keywords:
kinase D (PKD); diacylglycerol (DAG)

Materials & Methods:
Cells were seeded onto glass coverslips in 24-well plates in conjugation medium (RPMI medium without phenol red/0.5 % FCS) and allowed to attach for 20 min at 37ºC. T cells were treated with either 200 nM phorbol-1213-dibutyrate (PdBu Calbiochem) or

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Jurkat