Technical Reference #416

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
cover glass lining the 14-mm well of a 35-mm petri dish (MatTek Corporation; Ashland; MA)

416.

Imaging Beta Cell Development In Real-Time Using Pancreatic Explants From Mice With Green Fluorescent Protein–Labeledpancreatic Beta Cells SUBHADRA C. GUNAWARDANA; MANAMI HARA; GRAEME I. BELL; W. STEVEN HEAD; MARK A. MAGNUSON; AND DAVID W. PISTON, Vanderbilt University, In Vitro Cellular Developmental Biology- Animal, 41(416), (2005)
Link To Paper

Abstract:
We present a convenient method for monitoring pancreatic beta cell development in real-time through in vitro cultureof embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.

Keywords:
in vitro culture; pancreatic explants; mouse; GFP-labeled; real-time

Materials & Methods:
Pregnant mice were anesthetized terminally with an intraperitoneal injection of ketamine–xylazine (80:20 mg/kg). Embryos were removed from the uterus washed in ice-cold Hanks balanced salt solution (HBSS) and placed in ice-cold Roswell Park Memorial

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Egg