Technical Reference #415

415.

Definition Of Distinct Compartments In Polarized Madin–Darby Canine Kidney (Mdck) Cells For Membrane-Volume Sorting; Polarized Sorting Andapical Recycling Paul S. Brown; Exing Wang; Benjamin Aroeti; Steven J. Chapin; Keith E. Mostov and Kenneth W. Dunn, Indiana University, Traffic, 1(415), (2001)
Link To Paper

Abstract:
Previous studies of fibroblasts have demonstrated thatrecycling of endocytic receptors occurs through a defaultmechanism of membrane-volume sorting. Epithelial cellsrequire an additional level of polar membrane sortingbut there are conflicting mo

Keywords:
Endocytosis; endosome; epithelia; low density lipoprotein; MDCK; polarity; polymeric Ig receptor; transcytosis; transferrin

Materials & Methods:
As mentioned previously cells were grown on the underside of Millipore filter units. After removing the legs of the filter units living or fixed cells were observed by placing the entire filter unit on two 50-mm tape spacers attached to the coverslip

Microscopic Technique
Inverted Microscopy

Cell Type(s)
MDCK