Technical Reference #413

413.

Frequency Modulation Of Synchronized Ca2 Spikes In Cultured Hippocampal Networks Through G-Protein-Coupled Receptors Zhijun Liu; Lin Geng; Ruxin Li; Xiangping He; James Q. Zheng; and Zuoping Xie, Tsinghua University, Biochemistry Journal, 23(413), (2003)
Link To Paper

Abstract:
Synchronized spontaneous Ca2 spikes in networked neurons represent periodic burst firing of action potentials which are believed toplay a major role in the development and plasticity of neuronal circuitry. How these network activities are shaped and m

Keywords:
cAMP; neuropeptide; chemokine; synaptic transmission; Ca2 imaging; Ca2 oscillation

Materials & Methods:
Hippocampal neurons from embryonic rats (E18) were obtained according to the method previously described (Banker and Cowan 1977). In brief hippocampal tissues from 18 d fetal rats were dissected and treated with 0.25% trypsin and 0.25 mg/ml DNase (Si

Microscopic Technique
Inverted Microscopy

Cell Type(s)
OK