Technical Reference #411

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm culture dishes with glass bottoms (MatTek; Ashland; MA)

411.

Mek Inhibitor Pd98059 Acutely Inhibits Synchronized Spontaneous Ca2+ Oscillations In Cultured Hippocampal Networks Yan-fang RUI; Zhao-hui SUN; Jia-ping GU; Zhong-hua SHENG; Xiang-ping HE; Zuo-ping XIE, Tsinghua University, Acta Pharmacologica Sinica, 27(411), (2006)
Link To Paper

Abstract:
Aim: To investigate the changes in synchronized spontaneous Ca2+ oscillations induced by mitogen-activated protein kinase kinase (MEK) inhibitor PD98059 at different concentrations in cultured hippocampal network. Methods: Hippocampal neurons in culture f

Keywords:
PD98059; MEK; calcium oscillation

Materials & Methods:
Hippocampal neurons from embryonic rats (E18) were obtained according to the method previously described[25]. In brief hippocampal tissues from 18-d-old fetal rats were dissected and treated with 0.25% trypsin in Ca2+-Mg2+-free HBSS at 37 oC for 15 min;

Cell Type(s)
E18