Technical Reference #404

404.

Overexpression Of The Human Mnb/Dyrk1A Gene Induces Formation Of Multinucleate Cells Through Overduplication Of The Centrosome Eishi Funakoshi; Takamitsu Hori; Tokuko Haraguchi; Yasushi Hiraoka; Jun Kudoh; Nobuyoshi Shimizu and Fumiaki Ito, Setsunan University, BMC Cell Biology, 4(404), (2003)
Link To Paper

Abstract:
Previously we cloned the human MNB/DYRK1A gene from the "Down syndrome critical region" on chromosome 21. This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But the functi

Keywords:
overduplication; chromosome 21; autophosphorylation; Down syndrome

Materials & Methods:
The procedure for preparation of fluorescently-stained living cells for microscopic observation was described previously [3435]. Briefly HeLa cells were plated on a 35-mm glass-bottom culture dish (MatTek Corp. Ashland MA) and cultured for 1 day in a

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa