Technical Reference #403

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass coverslip at the bottom of a microwell dish (35 mm dish, poly-D-lysine-coated; MatTek, Ashland, MA, USA)

403.

A Combination Of Selective Cox-2 Inhibitors And Hydrogen Peroxide Increase The Reactive Oxygen Species Formation In Osteosarcoma Cells After X-Ray Irradiation TOSHIAKI TAKAHASHI, YASUHIRO OGAWA, TOSHIHIRO KOBAYASHI, HIROSHI SONOBE, HARUMICHI SEGUCHI, TOSHIKAZU TANI and SHOJI YOSHIDA, Kochi University, International Journal of Molecular Medicine, 14(403), 406, (2004)
Link To Paper

Abstract:
We investigated whether a combination of selective COX-2 inhibitors and hydrogen peroxide increases the effect of X-ray irradiation, with regard to reactive oxygen species (ROS) formation in an osteosarcoma cell line. COX-2 inhibitor did not induce ROS fo

Keywords:
COX-2 inhibitors, hydrogen peroxide, X-ray irradiation,

Materials & Methods:
Succinimidyl ester of H2DCFDA was obtained from Molecular Probes (Eugene, OR, USA). All other reagents were of the highest purity grade available. A stock solution of H2DCFDA was prepared in dimethyl sulfoxide (DMSO) and stored at -20°C. HS-Os-1 cells (1x

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HS-Os-1