Technical Reference #403

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass coverslip at the bottom of a microwell dish (35 mm dish; poly-D-lysine-coated; MatTek; Ashland; MA; USA)

403.

A Combination Of Selective Cox-2 Inhibitors And Hydrogen Peroxide Increase The Reactive Oxygen Species Formation In Osteosarcoma Cells After X-Ray Irradiation TOSHIAKI TAKAHASHI; YASUHIRO OGAWA; TOSHIHIRO KOBAYASHI; HIROSHI SONOBE; HARUMICHI SEGUCHI; TOSHIKAZU TANI and SHOJI YOSHIDA, Kochi University, International Journal of Molecular Medicine, 14(403), (2004)
Link To Paper

Abstract:
We investigated whether a combination of selective COX-2 inhibitors and hydrogen peroxide increases the effect of X-ray irradiation with regard to reactive oxygen species (ROS) formation in an osteosarcoma cell line. COX-2 inhibitor did not induce ROS fo

Keywords:
COX-2 inhibitors; hydrogen peroxide; X-ray irradiation;

Materials & Methods:
Succinimidyl ester of H2DCFDA was obtained from Molecular Probes (Eugene OR USA). All other reagents were of the highest purity grade available. A stock solution of H2DCFDA was prepared in dimethyl sulfoxide (DMSO) and stored at -20°C. HS-Os-1 cells (1x

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HS-Os-1