Technical Reference #400

400.

Potentiation Of Neurite Outgrowth And Reduction Of Apoptosis By Immunosuppressive Agents: Implications For Neuronal Injury And Transplantation JASON SHEEHAN; ANNE EISCHEID; RANDI SAUNDERS; AND NADER POURATIAN, University of Virginia, Neurosurgical focus, 19(400), (2006)
Link To Paper

Abstract:
Object: Immunosuppressive agents are believed to play a role in recovery from spinal cord injury but the underlying mechanisms by which neuronal function is improved by these agents are poorly understood. In this study the authors evaluate the effect of

Keywords:
spinal cord injury; immunosuppressive agent; apoptosis; calcium

Materials & Methods:
Undifferentiated SH-SY5Y cells (American Type Culture Collection Manassas VA) at passage numbers between 93 and 100 were plated in 35-mm glass-bottom dishes (MatTek Corp. Ashland MA) at densities of between 2 and 4 3 104 cells/dish in high-glucose DME

Microscopic Technique
phase Contrast Photomicrographs

Cell Type(s)
SH-SY5Y