Technical Reference #398

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
bottom glass microwell dish (MatTek Corp.; Ashland; MA)

398.

Rapid Diagnosis And Quantification Of Herpes Simplex Virus With A Green Fluorescent Protein Reporter System Szu-Hao Kung; Yu-Chun Wang; Chi-Hung Lin; Rei-Lin Kuo; Wu-Tse Liu, National Yang-Ming University, Journal of Virological Methods, 90(398), (2000)

Abstract:
A genetically modified cell line (Vero–ICP10-EGFP) was constructed for detection of herpes simplex virus (HSV) by a simple rapid and direct method.

Keywords:
Herpes simplex virus; Green fluorescent protein; ICP10 promoter

Materials & Methods:
For direct visualization under an inverted fluorescence microscope (Leica DMIRB) Vero– ICP10-EGFP cells were seeded on a bottom glass microwell dish (MatTek Corp. Ashland MA) a day before infection. Cells were infected with HSV in DMEM without phenol r

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Vero