Technical Reference #396

396.

Differential Gel Electrophoresis And Transgenic Mitochondrial Calcium Reporters Demonstrate Spatiotemporal Filtering In Calcium Control Of Mitochondria Selim Terhzaz; Tony D. Southall; Kathryn S. Lilley; Laura Kean; Adrian K. Allan; Shireen A. Davies and Julian A. T. Dow, University of Glasgow, The Journal of Biological Chemistry, 281(396), (2006)
Link To Paper

Abstract:
Mitochondria must adjust both their intracellular location and their metabolism in order to balance their output to the needs of the cell. Here we show by the proteomic technique of time series difference gel electrophoresis that a major result of neuroen

Keywords:
gel electrophoresis; neuropeptide signalling

Materials & Methods:
Adult flies were dissected in Schneider’s Drosophila medium (Invitrogen). For vital imaging of V-ATPase expression with the vhaSFD::GFP gene trap line (5) tubules were allowed to adhere lightly to a poly-Llysine treated coverslip glued to a window in the

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Drosophila