Technical Reference #390

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-l-lysine coated Mattek dish

390.

Gene Expression Analysis On Small Numbers Of Invasive Cells Collected By Chemotaxis From Primary Mammary Tumors Of The Mouse Weigang Wang; Jeffrey B Wyckoff; Yarong Wang; Erwin P Bottinger; Jeffrey E Segall and John S Condeelis, Albert Einstein College of Medicine of Yeshiva University, BioMedCentral Biotechnology, 3(390), (2003)
Link To Paper

Abstract:
cDNA microarrays have the potential to identify the genes involved in invasion and metastasis. However when used with whole tumor tissue the results average the expression patterns of different cell types. We have combined chemotaxis-based cell collecti

Keywords:
cDNA microarrays; breast cancer; metastasis

Materials & Methods:
Cells were collected from MMTV-PyMT mice. The needle contents were extruded into a 500 µl eppendorf tube with PBS/BSA/EDTA and diluted to 100 µl in the same buffer. 10 µl was removed and stained with DAPI to get an approximate cell count. The other 90 µl

Microscopic Technique
Laser Capture Microdissection

Cell Type(s)
MTLn3-GFP