Technical Reference #389

389.

Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates John N. Mason; Ian D. Tomlinson; Sandra J. Rosenthal; and Randy D. Blakely, Vanderbilt University, NanoBiotechnology Protocols - Methods in Molecular Biology, 303(389), (2008)
Link To Paper

Abstract:
The quantum dot is a novel fluorescent platform that has the potential to become an alternative to conventional organic dyes used to label biological probes such as antibodies or ligands. Compared to typical fluorescent organic dyes cadmium selenide/zinc

Keywords:
Streptavidin; quantum dots; biotinylation; cell-surface proteins; imaging

Materials & Methods:
Plate the dissociated cells on a poly-D-lysine and laminin-coated cover glass of a MatTek culture dish at a density of 20000 cells and incubate in a 5% CO2 incubator at 37°C. After 3 h flood the plates with 1 mL of growth medium. After 3 d of culturing

Microscopic Technique
Inverted Microscopy

Cell Type(s)
HEK-293