Localized Transfection With Magnetic Beads Coated With Pcr Products And Other Nucleic Acids
Maria Isabel Santori; Cayetano Gonzalez; Luis Serrano & Mark Isalan,
European Molecular Biology Laboratory,
Nature Protocols,
1(385),
(2006)
Link To Paper
Abstract:
The bead transfection method involves binding nucleic acids onto 3-μm-diameter paramagnetic beads treating the beads with transfection reagent and using them as scaffolds to direct transfection to individual cells or regions in a population. Typically Keywords:
bead transfection method; transfection microarray Materials & Methods:
Alternatively for MatTek dishes and plates fix the optically transparent base over the magnets with adhesive tape. Cover with 3 ml complete fresh DMEM medium with 10% FCS and 50 U ml–1 penicillin and 50 mg ml–1 streptomycin. Carefully pipette 1 ml of co Microscopic Technique
Fluorescence Microscopy Cell Type(s)
HEK-293 | 
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