Technical Reference #384

384.

A Paracrine Loop Between Tumor Cells And Macrophages Is Required For Tumor Cell Migration In Mammary Tumors Jeffrey Wyckoff, Weigang Wang, Elaine Y. Lin, Yarong Wang, Fiona Pixley, E. Richard Stanley, Thomas Graf, Jeffrey W. Pollard, Jeffrey Segall, and John Condeelis, Albert Einstein College of Medicine of Yeshiva University, Cancer Research, 64(384), 7023, (2004)
Link To Paper

Abstract:
Invasion of tumor cells into the surrounding connective tissue andblood vessels is a key step in the metastatic spread of breast tumors.Although the presence of macrophages in primary tumors is associatedwith increased metastatic potential, the mech

Keywords:
Mammary Tumors, epidermal growth factor (EGF), colony-stimulating factor 1 (CSF-1), metastasis, macrophage

Materials & Methods:
Cells collected into needles were extruded into a poly-L-lysine–coated MatTek dish (MatTek Corp., Ashland, MA) containing 20 L of 10% paraformaldehyde and fixed for 30 minutes. Nonspecific binding was blocked with 100 L Tris-buffered saline (TBS) wit

Microscopic Technique
Multi-photon Microscopy

Cell Type(s)
MTLn3