Technical Reference #384

384.

A Paracrine Loop Between Tumor Cells And Macrophages Is Required For Tumor Cell Migration In Mammary Tumors Jeffrey Wyckoff; Weigang Wang; Elaine Y. Lin; Yarong Wang; Fiona Pixley; E. Richard Stanley; Thomas Graf; Jeffrey W. Pollard; Jeffrey Segall; and John Condeelis, Albert Einstein College of Medicine of Yeshiva University, Cancer Research, 64(384), (2004)
Link To Paper

Abstract:
Invasion of tumor cells into the surrounding connective tissue andblood vessels is a key step in the metastatic spread of breast tumors.Although the presence of macrophages in primary tumors is associatedwith increased metastatic potential the mech

Keywords:
Mammary Tumors; epidermal growth factor (EGF); colony-stimulating factor 1 (CSF-1); metastasis; macrophage

Materials & Methods:
Cells collected into needles were extruded into a poly-L-lysine–coated MatTek dish (MatTek Corp. Ashland MA) containing 20 L of 10% paraformaldehyde and fixed for 30 minutes. Nonspecific binding was blocked with 100 L Tris-buffered saline (TBS) wit

Microscopic Technique
Multi-photon Microscopy

Cell Type(s)
MTLn3