Technical Reference #383

383.

The Delivery Of Trna To Cultured Mammalian Cells Mediated By Peptide Transduction Domains Steven Adrian Spronk In, California Institute of Technology, Electronic Thesis- Caltech, (2006)
Link To Paper

Abstract:
In vivo incorporation of unnatural amino acids using nonsense suppression is a powerfultechnique to study proteins. However one challenge to the method is that the amount ofunnatural protein that can be produced is directly limited by the amount of u

Keywords:
amino acids; aminoacyl-tRNA; protein transduction domains (PTDs)

Materials & Methods:
CHO-K1 cells were grown in Ham’s F12 medium supplemented with fetal bovine serum (10%) glutamine penicillin and streptomycin. HEK293 cells were grown in DMEM with the same additives. The medium with the additives is referred to as “growth medium.” Ce

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
CHO