Technical Reference #382

382.

Macrophages Promote The Invasion Of Breast Carcinoma Cells Via A Colony-Stimulating Factor-1/Epidermal Growth Factor Paracrine Loop Sumanta Goswami; Erik Sahai; Jeffrey B. Wyckoff; Michael Cammer; Dianne Cox; Fiona J. Pixley; E. Richard Stanley; Jeffrey E. Segall; and John S. Condeelis, Albert Einstein College of Medicine of Yeshiva University, Cancer Research, 65(382), (2005)
Link To Paper

Abstract:
Previous studies have shown that macrophages and tumor cells are comigratory in mammary tumors and that these cell types are mutually dependent for invasion. Here we show that macrophages and tumor cells are necessary and sufficient for comigration and in

Keywords:
Colony-Stimulating Factor-1/Epidermal Growth Factor; chemotactic factor

Materials & Methods:
MTLn3-GFP and RAW264.7 (LR5) cells were cultured in a-MEM with 5% fetal bovine serum (FBS). BAC1.2F51.2F5 cells were cultured in a-MEM with 10% FBS and 36 ng/mL of Human recombinant CSF-1 (a gift from Chiron Corp. Emeryville CA). MTLn3-GFP (n = 5000

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
MTLN3