Technical Reference #381

381.

Hydrogen Peroxide-Induced Cell Death In Cultured Aplysia Sensory Neurons Chae-Seok Lim; Jong-Cheol Lee; Su Dong Kim; Deok-Jin Chang; Bong-Kiun Kaang, Seoul National University, Brain Research, 941(381), (2002)
Link To Paper

Abstract:
Widespread neuronal cell death occurs during normal development and as a result of pathological conditions in the nervous system of many organisms. In this study we investigated the cytotoxicity induced by H2O2 in Aplysia mechanosensory neurons which se

Keywords:
Aplysia; Neuronal cell death; Hydrogen peroxide; Membrane potential; Input resistance

Materials & Methods:
Approximately 50–150 neurons were plated on poly-L-lysine (Sigma Chemical Co. St. Louis MO) coated culture dishes (P50G-0-14-F) containing L15 media with filtered hemolymph (1:1). Pleural ganglia were also organotypically cultured on the culture dishes

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HASM