Technical Reference #380

380.

Erythromycin Inhibits Atp-Induced Intracellular Calcium Responses In Bovine Tracheal Epithelial Cells Mitsuko Kondo; Soichiro Kanoh; Jun Tamaoki; Hideki Shirakawa; Shunichi Miyazaki; and Atsushi Nagai, Tokyo Women's Medical College, American Journal of Respiratory Cell and Molecular Biology, 19(380), (1998)
Link To Paper

Abstract:
Erythromycin (EM) therapy is known to decrease airway secretion in chronic inflammatory airway diseasessuch as diffuse panbronchiolitis. Airway secretion is regulated by intracellular Ca21concentration([Ca21]i). To elucidate the intrac

Keywords:
Erythromycin (EM) therapy; diffuse panbronchiolitis; Clarithromycin; FK506; FK-BP

Materials & Methods:
Cells cultured on glass-bottomed petri dishes (P35GC-101; MatTek Co. Ashland MA) were washed with Hepes-buffered HBSS and loaded with 10 mM fura2-AM for 1 h.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
E18