Technical Reference #379

379.

Importin A Can Migrate Into The Nucleus In An Importin B- And Ran-Independent Manner Yoichi Miyamoto; Miki Hieda; Michelle T.Harreman; Masahiro Fukumoto; Takuya Saiwaki; Alec E.Hodel; Anita H.Corbett and Yoshihiro Yoneda, Osaka University, The EMBO Journal, 21(379), (2002)
Link To Paper

Abstract:
A classical nuclear localization signal (NLS)-containing protein is transported into the nucleus via the formation of a NLS-substrate/importin / complex. In this study we found that importin migrated into the nucleus without the addition of importin R

Keywords:
ATP; importin a; nuclear pore complex; nuclear transport; time-lapse experiment

Materials & Methods:
HeLa cells or Madin±Darby bovine kidney (MDBK) cells were incubated in DMEM (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and penicillin/streptomycin (100 IU/ml and 100 mg/ml respectively) at 37°C in a 5% CO2 atmosphere. Cult

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Mammalian