Technical Reference #377

377.

Calcium And Dairy Products Inhibit Weight And Fat Regain During Ad Libitum Consumption Following Energy Restriction In Ap2-Agouti Transgenic Mice Xiaocun Sun and Michael B. Zemel, University of Tennessee, The Journal of Nutrition, 134(377), (2004)
Link To Paper

Abstract:
We demonstrated previously that dietary calcium suppression of calcitriol reduces adipocyte Ca2+ suppresses lipogenesis and increases lipid utilization during energy restriction. Notably dairy calcium sources exert markedly greater effects. To determin

Keywords:
obesity; intracellular calcium; dairy

Materials & Methods:
Adipose tissue was first washed several times with HBSS minced into small pieces and digested with 0.8 g/L type I collagenase in a shaking water bath at 37°C for 30 min. Adipocytes were then filtered through sterile 500- m nylon mesh and cultured in

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Adipocytes