Technical Reference #376

376.

In Vivo Near-Infrared Fluorescence Imaging Of Integrin V 3 In Brain Tumor Xenografts Xiaoyuan Chen; Peter S. Conti; and Rex A. Moats, University of Southern California (USC), Cancer Research, 64(376), (2004)
Link To Paper

Abstract:
Noninvasive visualization of cell adhesion molecule v 3 integrin expressionin vivo has been well studied by using the radionuclide imagingmodalities in various preclinical tumor models. A literature survey indicatedno previous use of cyanine dyes a

Keywords:
cell adhesion molecule v 3; subcutaneous U87MG glioblastoma xenograft; Integrins v 3 and v 5; tumor growth and metastasis; tumor angiogenesis; arginine-glycine-aspartic acid (RGD) peptides

Materials & Methods:
For laser confocal microscopy cells grown on 35-mm MatTek imaging dishes (Cat no. P35G-0- 14-C Ashland MA) were washed with PBS and then were incubated at 37°C in the presence of 100 nmol/L RGD-Cy5.5 for 1 hour. Afterward cells were washed in ice-

Microscopic Technique
Confocal Microscopy

Cell Type(s)
U87MG