Technical Reference #374

374.

Cell Density And N-Cadherin Interactions Regulate Cell Proliferation In The Sensory Epithelia Of The Inner Ear Mark E. Warchol, Central Institute for the Deaf, The Journal of Neuroscience, 22(374), (2002)
Link To Paper

Abstract:
Sensory hair cells in the inner ears of nonmammalian vertebrates can regenerate after injury. In many species replacement hair cells are produced by the proliferation of epithelial supporting cells. Thus the ability of supporting cells to undergo renewe

Keywords:
auditory; vestibular; regeneration; hair cell; adhesion molecules; cell culture

Materials & Methods:
Isolated epithelia were then placed on a small spatula and transferred into fibronectin- or laminin-coated culture wells that contained 50 l of medium 199 (with Earle’s salts 2200 mg/ l sodium bicarbonate 25 mM HEPES and 0.69 mM L-glutamine) supp

Microscopic Technique
Optical Microscopy, Differential Interference Contrast Microscopy

Cell Type(s)
Sensory