Technical Reference #373

373.

Reduction In Dna Synthesis During Two-Photon Microscopy Of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence Michael G. Nichols; Erin E. Barth and Jennifer A. Nichols, Creighton University, Photochemistry and photobiology, 81(373), (2005)
Link To Paper

Abstract:
Two-photon laser scanning microscopy (TPLSM) of endogenous reduced nicotinamide adenine dinucleotide (NAD(P)H) provides important information regarding the cellular metabolic state. When imaging the punctate mitochondrial fluorescence originating from NAD

Keywords:
nicotinamide adenine dinucleotide (NAD(P)H); multiphoton excitation; near-infrared photodamage; metabolic imaging

Materials & Methods:
For a given experiment RBL cells were plated at a density of 1.15 x 10^5 cells/mL on glass coverslip-bottomed grid dishes (P35G-7-c-grid; MatTek Ashland MA) and allowed to adhere overnight. Adherent cells were exposed to intense near-infrared illuminat

Microscopic Technique
Two Photon Laser Scanning Microscopy (TPLSM)

Cell Type(s)
RBL-2H3