Technical Reference #369

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
no. 1.5 glass-bottomed chamber (catalog no. P35G-1.5–14-C; Mat-Tek; Ashland; MA)

369.

Relating Microstructure To Rheology Of A Bundled And Cross-Linked F-Actin Network In Vitro J. H. Shin; M. L. Gardel; L. Mahadevan; P. Matsudaira; and D. A. Weitz, Massachusettes Institute of Technology, PNAS, 101(369), (2004)
Link To Paper

Abstract:
The organization of individual actin filaments into higher-order structures is controlled by actin-binding proteins (ABPs). Although the biological significance of the ABPs is well documented little is known about how bundling and cross-linking quantitat

Keywords:
in vitro; scruin; actin; actin-binding protein; multiparticle tracking

Materials & Methods:
G-actin solutions were prepared by dissolving lyophilized G-actin in deionized water and dialyzing the solutions against fresh G-buffer (2 mM TrisHCl0.2 mM ATP0.2mMCaCl20.2mMDTT0.005% NaN3 pH8.0) at 4°C for 24 hr; the buffer was replaced with fr

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Eukaryotic