Technical Reference #367

367.

Using A Histone Yellow Fluorescent Protein Fusion For Tagging And Tracking Endothelial Cells In Es Cells And Mice Stuart T. Fraser; Anna-Katerina Hadjantonakis; Kenneth E. Sahr; Stephen Willey; Olivia G. Kelly; Elizabeth A.V. Jones; Mary E. Dickinson; and Margaret H. Baron, Mount Sinai School of Medicine, Genesis, 42(367), (2005)
Link To Paper

Abstract:
We report the first endothelial lineage-specific transgenic mouse allowing live imaging at subcellular resolution. We generated an H2B-EYFP fusion protein which can be used for fluorescent labeling of nucleosomes and used it to specifically label endothel

Keywords:
Flk1::H2B-EYFP; endothelial cells; fluorescent protein; EYFP; vasculogenesis; angiogenesis; imaging; transgenic mice; histone fusion

Materials & Methods:
The entire insert from pFlk1::H2B-EYFP containing Flk1 regulatory regions and the Flk1::H2B-EYFP-pA fragment was excised from pFlk1enh by digestion with SalI and XbaI and gel-purified for pronuclear injection into C57BL/6 C3H (B6C3) F1 hybrid embr

Microscopic Technique
Confocal Microscopy, Laser Scanning, Stereo Microscopy

Cell Type(s)
Mixed cortical cultures (neurons and glia)