Technical Reference #357

357.

Mechanism Of Prion Propagation: Amyloid Growth Occurs By Monomer Addition Amyloid Growth Occurs By Monomer Addition Sean R. Collins; Adam Douglass; Ronald D. Vale; Jonathan S. Weissman, HHMI, PLoS Biology, 2(357), (2004)
Link To Paper

Abstract:
Abundant nonfibrillar oligomeric intermediates are a common feature of amyloid formation and these oligomers rather than the final fibers have been suggested to be the toxic species in some amyloid diseases. Whether such oligomers are critical intermed

Keywords:
prion inheritance; polymerization; NM; a fragment of the Sup35 protein containing the glutamine/asparagine-rich N-terminal (N) and highly charged middle (M) domains; amyloid formation

Materials & Methods:
For continuous thioflavin T assay measurements concentrated stocks of NM stored in either 6 M GuHCl (fluorescently labeled NM and NM used for Figures 2C 2F and 6) or 4 M GuHCl and 4 M urea (NM used for all other experiments) were diluted at least 2

Microscopic Technique
Scanning Probe Microscopy, Atomic Force Microscopy (AFM)

Cell Type(s)
Coleoptiles