Technical Reference #356

356.

Ephb–Ephrinb Bi-Directional Endocytosis Terminates Adhesion Allowing Contact Mediated Repulsion Manuel Zimmer; Amparo Palmer; Jenny Köhler and Rüdiger Klein, Max-Planck Institute, Nature Cell Biology, 5(356), (2003)
Link To Paper

Abstract:
Eph receptors and their membrane-associated ephrin ligands mediate cell−cell repulsion to guide migrating cells and axons. Repulsion requires that the ligand−receptor complex be removed from the cell surface for example by proteolytic processing of the e

Keywords:
endocytosis; eph receptors; ephrin ligands; ligand-receptor complex; adhesion; cell repulsion;

Materials & Methods:
Kinase deficient mouse EphB2 was generated by site directed mutagenesis leading to exchange of Lys 660 to Arg. To generate EphB2–YFP (pJK12) EYFP was cloned in frame into the juxtamembrane region of Flag–EphB2. NIH3T3 cells stably expressing full-leng

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa