Technical Reference #353

353.

Regulation Of Meiotic Prophase Arrest In Mouse Oocytes By Gpr3; A Constitutive Activator Of The Gsg Protein Leon Freudzon; Rachael P. Norris; Arthur R. Hand; Shigeru Tanaka; Yoshinaga Saeki; Teresa L.Z. Jones; Mark M. Rasenick; Catherine H. Berlot; Lisa M. Mehlmann; and Laurinda A. Jaffe, University of Connecticut, The Journal of Cell Biology, 171(353), (2005)
Link To Paper

Abstract:
The arrest of meiotic prophase in mouse oocytes within antral follicles requires the G protein Gs and an orphan member of the G protein–coupled receptor family GPR3. To determine whether GPR3 activates Gs the localization of Gαs in follicle-enclosed ooc

Keywords:
meiotic prophase; GPR3; somatic cells; cAMP; immunoblotting; oocyte

Materials & Methods:
Ovaries were collected from 20–25-d-old mice. Preantral follicles and early antral/antral follicles were dissected from ovaries. 4–10 follicles were placed on Millicell culture plate inserts inside coverslip-bottomed dishes containing 1.6 ml of medium. Th

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Cranial neural crest