Technical Reference #351

351.

Process Of Apoptosis Induced By Tnf-A In Murine Fibroblast Ltk-Cells: Continuous Observation With Video Enhanced Contrast Microscopy K. Okamoto; M. Mizuno; N. Nakahara; A. Natsume; J. Yoshida; T. Mori; S. Hori and H. Kobayashi, Nagoya University, Apoptosis, 7(351), (2002)
Link To Paper

Abstract:
Apoptosis Is originally defined by unique morphological changes of dying cells and the biochemical hallmark associated with apoptosis is internucleosomal DNA fragmentation. However few report has shown the precise time course of the apoptotic events. Th

Keywords:
apoptosis; caspase; Dnase ?; Ltk-; TNF-a; VEC-DIC microscopy

Materials & Methods:
The murine fibroblast cell line Ltk- which is highly sensitive to TNF-a was maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum 0.1 mM nonessential amino acids 5 mM L-glutamine and antibiotics (streptomycin

Microscopic Technique
Video Enhanced Contrast Differential Interference Contrast (VEC-DIC)

Cell Type(s)
Ltk-cells