Technical Reference #350

350.

In Vivo Evidence For Short- And Long-Range Cell Communication In Cranial Neural Crest Cells Jessica M. Teddy; Paul M. Kulesa*, Stowers Institute for Medical Research, Development, 131(350), (2004)
Link To Paper

Abstract:
The proper assembly of craniofacial structures and theperipheral nervous system requires neural crest cells toemerge from the neural tube and navigate over longdistances to the branchial arches. Cell and molecularstudies have shed light on potenti

Keywords:
chick; neural crest; cranial; filopodia; cell guidance; confocal; time-lapse imaging; cell differentiation

Materials & Methods:
Fertile white leghorn chick eggs were acquired from a local supplier and incubated at 38°C until approximately the 5- 8 somite stage (ss) of development. Eggs were rinsed with 70% alcohol and 3 ml of albumin was removed before cutting a window in the s

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Embryos